Measures human tissue factor (TF, Thromboplastin, Factor III) in plasma, tissue extracts and cell culture supernatants.
Tissue factor (TF) is membrane-bound glycoprotein that serves as both the receptor and cofactor for Factor VII (fVII) and Factor VIIa (fVIIa) in initiating cell surface procoagulant activity. TF is absent on cells within the vascular endothelium or, if present, its conformation is not suitable to react with Factor VII. However, when tissue injury disrupts the vascular endothelium, the barrier separating intravascular factor VII from TF is broken and Factor VII/TF complexes can be formed. The TF/fVIIa complex activates Factors IX and X by limited proteolysis, leading ultimately to thrombin generation and fibrin formation. When monocytes, macrophages and endothelial cells are stimulated by endotoxins, cytokines and lectins, TF is upregulated and an increase in procoagulant activity is seen. This assay recognizes TF-apo, TF and TF/fVII complexes.
For Research Use Only
|PRODUCT NAME||REF||# of Tests|
|IMUBIND® Tissue Factor ELISA||845||96|